登革热媒介白纹伊蚊mtDNA-ND4基因特征分析

mtDNA-ND4 gene analysis of dengue vector Aedes albopictus

目的研究不同地理种群白纹伊蚊(Aedes albopictus)线粒体蛋白NADH脱氢酶亚基Ⅳ(mtDNA-ND4)基因多态性,探讨其遗传特征。方法采集不同海拔高度、登革热历史流行区与非流行区的白纹的幼虫,实验室饲养至成蚊,PCR扩增mtDNA-ND4基因并遗传分析。结果获mtDNA-ND4长度为324b,碱基A+T含量分别为73.2%,17个碱基置换位点,颠换率为62.5%;19个个体存在5个单倍型,单倍型多态性(h)为0.442。结论福建省不同地理株白纹伊蚊出现小程度的遗传分化,可能由遗传漂变引起的。

In order to study the characteristics of mitochondrial DNA NADH dehydrogenase subunit 4 （mtDNA-ND4） gene and to understand and propose genetic polymorphism of vectors, mosquito larvae were collected from dengue epidemical and non-endemic areas at different altitude levels,and to rear to adult mosquitoes. Together with Aedes albopictus from other regions （Yunnan, Shanghai and Guizhou） in China, their mtDNA-ND4 genes were amplified and the gene sequences were obtained and analyzed. Results revealed that the ND4 fragment contained 324 nucleotides with 17 polymorphie sites. The A＋T content of ND4 gene was 73.2 %. The frequency of transversion was 62.5 % and 5 haplotypes were obtained from 19 specimens among 4 geographic populations from Fuiian Province. The haplotype diversity was 0. 442. All results showed that genetic difference existed among population Of strains, while the degree was lower. This difference might be caused by genetic drift.