辛伐他汀对高糖诱导乳鼠心肌细胞过氧化损伤的保护作用

The protective effects of simvastatin on high glucose-induced over-oxidation injury of the neonatal rat cardiomyocytes

目的探讨辛伐他汀对高糖所致乳鼠心肌细胞氧化应激损伤的保护作用。方法分离SD乳鼠心肌细胞原代培养72h后，随机分为5组并加入相应的处理药物，即对照组（control组）；高浓度葡萄糖刺激组（GS组）；不同浓度的辛伐他汀干预组，分别为GS＋10^-7MSim组、GS＋10^-6MSim组和GS＋10^-5MSim组。测定各组心肌细胞活力及心肌细胞培养基中丙二醛（MDA）含量、超氧化物歧化酶（SOD）活力及细胞内总谷胱甘肽（GSH）含量。结果辛伐他汀呈剂量依赖性地提高高糖损伤乳鼠心肌细胞的细胞活力（P〈O．01）；培养基中MDA的生成减少（P〈0．05），而SOD活力及GSH含量明显增高（P〈0．05）。结论辛伐他汀可能通过稳定细胞膜、抗脂质过氧化反应及提高清除氧自由基，对高糖所致的心肌细胞过氧化损伤发挥剂量依赖性的保护作用。

Objective To investigate the protective effects of simvastatin on cardiomyocytes injury induced by high concentration of glucose in the neonatal rats. Methods Ventricular myocytes were isolated from hearts of the neonatal SD rats by enzymatic digestion and anchorage velocity-dependent separation method for primary culture. Cardiomyocytes were randomly divided into five groups： control group, high glucose group（GS）, different concentration simvastatin （10^-7 mol/L, 10^-6 mol/L, 10^-5 mol/L）＋high glucose group. The following measures were detected： cadiocyte viability, activities of superoxide dismutase （SOD）, the contents of malondialdehyde （MDA） and glutathione（GSH） respectively. Results Simvastatin could protect the myocardial cells from high glu- cose injury in a dose-dependent manner, and Simvastatin（ 10^-5 mol/L） preconditioning showed the greatest protective effect. Therefore, simvastatin preconditioning could significantly attenuate the increased MDA level and antagonize the reduced SOD activity and contents of GSH（P〈0.05）. Conclusion Simvastatin might be has the protective effects on cardiomyocytes injured by high concentration glucose through improving cell antioxidant ablility.