运动性疲劳影响大鼠学习记忆功能和脑组织内Tau的表达

Effects of Exercise-induced Fatigue on Rat Learning and Memory Ability and Expressions of Tau in the Brain

目的:建立大鼠运动性疲劳模型,通过免疫组织化学法检测大鼠大脑皮层和海马CA1区内Tau蛋白的分布与表达,探讨其在运动性疲劳致大鼠学习记忆障碍发病机理中的作用。方法:成年雄性Wistar大鼠随机平均分为对照组、疲劳即刻组和疲劳恢复90 min组。疲劳即刻组和疲劳恢复90 min组大鼠进行十天游泳运动,每天运动至力竭,对照组大鼠不加干预。运用避暗穿梭实验检测大鼠学习记忆功能损伤情况,免疫组织化学法检测大脑皮层和海马CA1区Tau蛋白分布及表达,并测定Tau蛋白免疫阳性细胞的积分光密度值(IOD)。结果:疲劳即刻组和疲劳恢复90 min组大鼠学习记忆功能有不同程度损伤,其大脑皮层和海马CA1区Tau蛋白表达较对照组上调(P<0.05)。结论:运动性疲劳致大鼠学习记忆功能损伤可能与大鼠大脑皮层和海马内Tau蛋白的表达变化相关。

Objective： Through establishing the rat model of exercise-induced fatigue,the paper detected the distribution and expression of Tau in the cerebral cortex and hippocampus CA1,and explored the role of Tau in fatigue-induced learning and memory disorder.Methods： Adult male SD rats were randomly divided into control group,10-day fatigue group and recovery 90min group.Rats in 10-day fatigue group and recovery 90min group exercised to exhausted by swimming for 10 days,and those in control group didn＇t exercise.Dark avoidance test was used to detect the changes of learning and memory ability.Immunohistochemistry was used to observe the distribution and expressions of Tau in the cerebral cortex and hippocampus.IOD of Tau-immunoreactive cells were also countered.Results： Rats with exercise-induced fatigue showed learning and memory disorder,and in the cerebral cortex and hippocampus CA1 of rats in the fatigue group,the expressions of Tau increased obviously compared with that of the control group（P0.05）.Conclusions： The present results suggest that the learning and memory disorder induced by fatigue might relate to the changes of the expression of Tau in the cerebral cortex and hippocampus CA1.