树龄对毛橘红药材中黄酮类物质含量的影响

Study on the effect of tree age on total flavonoids in Citri grandis

目的研究树龄对毛橘红药材中总黄酮和柚皮苷含量的影响规律。方法采用紫外-可见分光光度法测定从不同树龄化州柚母树所采毛橘红果皮中的总黄酮含量;采用HPLC法测定从不同树龄毛橘红母树所采毛橘红果皮中柚皮苷含量。结果总黄酮含量以4年生毛橘红为最高,达23.78%;6年生毛橘红最低,只有10.48%。柚皮苷含量以4年生毛橘红为最高,达15.53%;6年生毛橘红最低,只有6.42%。结论树龄对同一生产区毛橘红药材总黄酮和柚皮苷含量有一定的影响,以4年生含量最高。4年生母树产毛橘红样品总黄酮含量显著高于3年生者;但随着树龄的继续增加,总黄酮和柚皮苷含量呈下降趋势。

Objective To study on the effect of tree age on total flavonoids in Citri grandis.Methods The contents of total flavonoids were determined with ultraviolet spectrophotometric method and naringin was assayed with HPLC in fruits from different years of Citrus grandis ’tomentosa’.Results The contents of total flavonoids and naringin were 23.78% and 15.53% respectively,which were the highest in the fruits of Citri grandis collected from 4 years old mother trees.However,the results were 10.48% and 6.42% respectively,the lowest in the fruits collected from 6 years old mother trees.Conclusion The age of mother tree may affect the contents of total flavonoids and naringin in fruits from Citri grandis of the same origin.The contents are highest in the fruits collected from 4 years old mother trees.But they may decline with the growth of tree age in the fruits from 5 and 6 years old trees.