设施番茄植株中百菌清和毒死蜱残留分析方法研究

Analytical Method for Determining Residues of Chlorothalonil and Chlorpyrifos in Greenhouse Tomato Plant

运用超声提取、氧化铝-硅胶组合填料层析净化、气相色谱-质谱联用(GC-MS)检测,建立了设施番茄植株根、茎、叶和果实中同时测定百菌清和毒死蜱含量的分析方法。针对设施番茄植株叶绿素含量高的特点,优化了层析柱填料和洗脱溶剂配比,并研究了两种农药在层析柱中的洗脱规律。结果表明,在番茄根、茎、叶和果实中,百菌清平均回收率分别为95.9%、98.2%、86.2%和98.1%,毒死蜱的平均回收率分别为105.4%、99.5%、97.0%和98.0%。百菌清和毒死蜱的最低检测限分别为0.028 mg/kg和0.033 mg/kg。对比色素对两种农药在层析柱上洗脱的影响发现,大量叶绿素降低了百菌清的回收率。

Analytical method was developed with the purpose to determine residues of two commonly used insecticides,i.e, Chlorothalonil and Chlorpyrifos,in tomato plants grown in greenhouse,which involved the application of ultrasonic extraction,chromatographic purification with mixed fillings of alumina and silica gel and GC-MS detection.Considering high level of chlorophyll in tomato plants,elution of the two pesticides through chromatographic columns was optimized in terms of chromatographic fillings and elution solvents.As a result,average recoveries of chlorothalonil and chloropyrifos in tomato leaves,roots,stems and fruits respectively were obtained.By comparing the effects of chlorophyll on elution of two pesticides,the authors found that high chlorophyll in the tomato plants caused reduction of recovery of chlorothalonil.