大白猪育种核心群氟烷基因PCR-RFLP检测及其净化

Identification and eliminatation of halothane gene in core breeding groups of large white pig by PCR-RFLP

采用PCR-RFLP技术,对正邦集团大白猪原始基础群及其育种核心群0、1世代共538头个体,以及红星农场大白猪原始基础群和育种核心群0世代共249头个体进行氟烷基因(Hal)基因型检测,并对阳性个体予以清除使其净化。基因继代选择结果显示,正邦原始基础群及核心群0、1世代群体Hal n基因频率分别为9.48%、2.22%、0;红星原始基础群及核心群0世代群体Hal n基因频率分别为1.08%、0,呈现世代递减并实现净化,进而培育出大白猪抗应激品系。研究表明,PCR-RFLP技术可准确诊断猪氟烷基因,在种猪选育过程中及时淘汰携带氟烷基因的个体,提高猪种质量。

Herd breeding is a method used widely in pig production. PCR-RFLP was employed to identify the halo thane genotype of breeding group pigs. Totally,538 large white pigs of original foundation group and breeding core groups of primary and first（0.1） generations from Zhengbang company and 249 large white pigs of original founda tion group and core group primary （0） generation from Red star farm were concluded in this study. Then halothane positive individuals were eliminated based the result of genotyping. The result indicated that the Hal^n gene frequency of original foundation group and breeding core groups of primary and first （0, 1） generation was 9.48 %, 2.22 % and 0% from Zhengbang company and 1.08% .0% from Red star farm. It showed that gene halothane was reduced grad ually from generation to generation and purified completely, and cultivated anti-stress strains large white pigs finally. It showed that halothane gene could be tested definitely by PCR-RFLP technology,and halothane positive individuals could he eliminated in swine selection progress, this technology will useful and helpful in improving the quality of pigs.