摘要
利用流式细胞仪及ELISA技术,通过对血液中CD4+、CD8+和血液及子宫分泌物中三种免疫球蛋白(IgA、IgG、IgM)含量进行检测,探讨在炎症状态下,各指标的动态变化与诊断子宫内膜炎的相关性。结果表明,在感染后期CD8+占主导地位,CD4+/CD8+也随之降低。而产后奶牛血液中IgA、IgG、IgM均呈现升高趋势(P<0.05,P<0.01),表明细胞免疫降低时,体液免疫正在加强,且临床型子宫内膜炎(CE)组变化比隐性子宫内膜炎组(SE)明显。
Using flow cytometry and ELISA method to detect the concentration of CD4＋,CD8＋ in blood and three immunoglobulins（IgA,IgG,IgM） in serm and in uterine secretion.The objective was to study the associativity between dynamic state change of each index and diagnosing endometritis.The results showed that CD8＋ had the leading position in the later period,and CD4＋/CD8＋ was descent.The content of IgA,IgG and IgM in peripheral blood with infected dairy cows increased significantly（P0.05 or P0.01） and humoral immunity was activated in the infected cows when cell mediated immunity was lowered and that of CE group was variable more significantly than that of SE group.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2011年第6期86-90,共5页
Journal of Northeast Agricultural University
基金
国家科技支撑计划子课题(2006BAD04A05)
