MyD88、TLR4和STAT3在肝细胞癌组织中的表达及意义

Expression and significance of MyD88,TLR4 and STAT3 in hepatocellular carcinoma

目的探讨TLR4、MyD88、STAT3在肝细胞癌组织中的表达及生物学意义。方法采用免疫组化方法检测TLR4、MyD88、STAT3在82例肝癌组织及其对应的癌旁肝组织中的表达水平。结合肝癌临床病理指标分析相关性。结果 TLR4、MyD88、STAT3蛋白在癌组织中的阳性表达率为76.8%(63/82)、67.1%(55/82)、69.5%(57/82),高于在癌旁肝组织中的阳性表达率25.9%(12/82)、12%(9/82)、8.5%(7/82),差异均具有统计学意义(P<0.05);在肝癌组织中TLR4、MyD88、STAT3的阳性表达呈正相关[TLR4和MyD88(r=0.612,P=0.011),MyD88和STAT3(r=0.578,P=0.002),TLR4和STAT3(r=0.542,P=0.016)]。MyD88和STAT3表达与性别、分化程度、有无HBV感染和肝硬化有关(P<0.05),而与肿瘤大小、有无静脉浸润无关(P>0.05)。结论 TLR4、MyD88、STAT3表达上调,导致肝癌细胞增殖和免疫逃逸是肝癌发生发展的重要分子机制。

【Objective】 To investigate the expression and significance of TLR4,MyD88 and STAT3 in hepatocellular carcinoma.【Methods】 S-P immunohistological assay was applied to detect the expression of TLR4,MyD88 and STAT3 genes in hepatocellular carcinoma tissues and adjacent normal tissues of 82 cases.Correlation analysis was used to determine the relation of TLR4,MyD88 and STAT3 with clinicopathological data.【Results】 The positive rates of TLR4,MyD88 and STAT3 expression were 76.8%（63/82）,67.1%（55/82） and 69.5%（57/82） in 82 hepatocellular carcinoma tissues,which were significantly higher than those（25.9 %,12% and 8.5 %） in adjacent normal tissues（P〈0.05）.TLR4 expression was positively correlated with MyD88（r =0.612,P =0.011） and STAT3（r =0.542,P =0.016）,MyD88 and STAT3 expression was also positively correlated（r =0.578,P =0.002）.The expression of MyD88 and STAT3 in HCC was in significant relation to gender,age,pathological grade,HBV infection and liver cirrhosis（P〈0.05）,but not significantly related to tumor size or venous infiltration（P〈0.05）.【Conclusions】 TLR4,MyD88 and STAT3 proteins are significantly over-expressed in HCC tissues.The over-expression results in cell proliferation and immune escape which promote the initiation and development of HCC.