不同方法提取鸦胆子挥发油化学成分的GC-MS分析

A Gas Chromatography-Mass Spectrometry Analysis of the Essential Oils from Brucea javanica Extracted with Different Methods

分别采用水蒸气蒸馏法(HD)和微波辅助水蒸气蒸馏法(MAHD)提取鸦胆子挥发油。运用毛细管气相色谱-质谱联用仪结合计算机检索对所得挥发油成分进行分析和鉴定,用气相色谱面积归一法测定各组分的相对质量分数。结果显示,两种方法分别从鸦胆子挥发油中鉴定出42种和37种化合物,分别占总峰面积的94.09%和94.13%。不同方法所得鸦胆子挥发油化学组成不同,但主要成分是黄樟脑、(+)-4-蒈烯、β-香叶烯、丁香油酚甲醚、2,6-二甲氧基甲苯、柠檬烯和甲基胡椒酚。

Brucea javanica（Simaroubaceae） is a medicinal plant traditionally used in Southern China to treat several diseases.In order to enlarge the medication scope,the constituents of the essential oils extracted from Brucea javanica with microwave-assisted hydrodistillation（MAHD） and hydrodistillation（HD） were analyzed by capillary GC-MS and elucidated based on the standard mass spectral data.42 and 37 kinds of volatile components were identified respectively.The relative mass fractions of the constituents were determined by area normalization method.The identified relative contents of the essential oils were 94.09% and 94.13%,respectively.There were differences in some chemical constituents between the two kinds of essential oils.However,their main constituents were safrole,（＋）-4-carene,β-myrcene,eugenol methyl ether,2,6-dimethoxy toluene,limonene and estragole.