响应曲面法优化超声辅助提取油菜籽皮中的原花青素

Optimization of Ultrasound-assisted Extraction of Proanthocyanidin from Rapeseed Hull Using Response Surface Methodology

以乙醇为提取剂,用超声辅助的方法从油菜籽皮中提取了原花青素。在讨论了提取剂体积分数、提取时间、液料比(每克原料加入提取剂的毫升数,简称液料比,以下同)和超声波功率等单因素实验的基础上,运用Box-Behnken中心组合实验和响应曲面法分析了提取剂体积分数、提取时间和液料比3个因素对原花青素提取率的影响,并优化了提取工艺。结果表明,最佳的工艺条件为:乙醇体积分数62.9%,提取时间20.8 min,液料比25 mL/g。在该条件下,原花青素提取率的预测值为5.055 mg/g,验证实验值为5.02 mg/g,说明响应曲面法优化油菜籽皮中原花青素提取工艺是可行的。

Ultrasound-assisted extraction of proanthocyanidin from rapeseed hull using ethanol as the extraction solvent was studied.Based on the single factor tests of ethanol volume fraction,extraction time,liquid-solid ratio and ultrasound power,the optimum process was evaluated by Box-Behnken central composite experimental design and response surface methodology analysis.The results indicate that ethanol volume fraction of 62.9%,extraction time of 20.8 min and liquid-solid ratio of 25 mL/g were found to be the optimum conditions under study.Under these optimized conditions,the experimental value of proanthocyanidin extraction yield was 5.055 mg/g,in exact accordance with the predicted value 5.02 mg/g.Therefore,the ultrasound-assisted extraction of proanthocyanidin from rapeseed hull using response surface methodology is feasible and reliable.