HPLC法测定五味子品种中木脂素含量

Determination of lignan content in Schisandra chinensis by HPLC

目的测定南五味子、北五味子、北五味子南移栽培品种中木脂素的含量。方法采用C18柱,(250×4.6mm,5μm),选用甲醇-水(75∶25)为流动相(流速:1.0 mL/min),检测波长为250nm。结果五味子醇甲、酯甲、甲素、乙素分别在0.073～0.73μg,0.147 5～1.475μg,0.064～0.64μg,0.134～1.34μg之间线性关系良好,相关系数分别为R=0.999 4,r=0.998 5,r=0.998 5,r=0.999 2,平均回收率为100.77%,RSD为0.5%。结论测定方法简便、快速、可靠,可以作为不同种五味子中木脂素含量的质量控制。

Aim To determine the concentration of lignans in different Schizandra chinesnis （Turcz.） Baill by HPLC. Methods A hypersil OSD column（250 ×4. 6 mm,5μm） was used with a mobile phase of methanol-water （75： 25）. Wave length was 250 nm. Results The standard curve of schizandrin was linear（ r = 0. 999 8 ） and the concentration range was from 0. 75 ug to 3.75 ug. The average recovery rate of schizandrin was 100. 77%, （ RSD = O. 5% ）. Conclusion This method is simple, rapid, reliable and suitable for measuring total lignans in Schizandra chinesnis（ Turcz. ） Baill.