苯酚—硫酸法测定玉米须保健饮料中多糖的含量

Determination of Total Polysaccharides in Corn Stigma Health Beverage by Phenol-sulfuric Acid Method

采用苯酚—硫酸法,以葡萄糖为对照品,于波长490 nm处测其吸光度,测定总多糖的含量。结果表明,葡萄糖质量浓度为0.02～0.14 mg/mL,与吸收度呈良好的线性关系;回归曲线:Y=5.201 8X-0.002 4,R2=0.999 6;平均回收率为100.55%,RSD=1.70%,测得的总多糖平均质量浓度为0.874 mg/mL。

Using the phenol - sulfuric acid method with the glucose as comparison, its absorption at 490 nm is measured, the total polysaccharide content is determined. The linear range of glucose is 0.02 - 0.1d mg/mL （R^2=0.999 6） with an average recovery of i00.55 % （RSD=1.70 %）. The assay Of total polysaccharides in corn stigma health beverage is 0.874 mg/mL.