用反相离子色谱法测定大麦草片剂中VB_1的含量

Determination of Ⅱ—Carotene in Damaicao Tablets by Ion-pair Reversed-phase High Performance Liquid Chromatography

建立大麦草片剂中VB1的反相离子色谱的测定方法。测定方法为:色谱柱:UltimateTM column XB-C18,5μm,4.6 mm×250 mm;流动相:0.05 mol/L乙酸钠溶液和甲醇的体积比为60∶40;流速:1.0 mL/min;检测波长:激发波长375 nm,发射波长435 nm。结果表明,VB1质量为0.000 1～0.000 5μg,线性关系良好(R2=0.999 8),平均回收率为99.44%。该方法简单、专属性强、灵敏度高,适用于大麦草片剂中VB1的含量测定。

A method for determination of VB, in Damaicao Tablets by Ion-pair reversed-phase high performance liquid chromatography is established. The Chromatographic conditions are that using UltimateTM column XB-C18 （5 μm, 4.6 mm× 250 mm） as chromatographic column, sodium alcoholate （0.05 tool/L） --methanol （60 ： 40） as mobile phase.The flow rate is 1.0 mL/min, its excitation and emission wavelength at 375 nm and 435 nm respectively. The method is proved to be linear in the ranger of 0.000 1 -0.000 5 μg （R^2=0.999 8）, the average recovery is 99.44%. The method is simple, exclusive, sensitive and reproducible. It is suitable for determination of VB1 in Damaicao Tablets.