利用H_2O_2和邻氨基酚伏安法测定HRP及HRP标记物(英文)

Determination of HRP and Labelled HRP with Voltammetry using Hydrogen Peroxide and O-Aminophenol

提出了邻氨基酚（OAP）－H2O2-辣根过氧化物酶（HRP）伏安酶联免疫分析新体系并用于测定HRP及HRP标注物。HRP催化H2O2氧化OAP生成的中间产物邻苯醌亚胺在BR缓冲液中，在-0．87V（vs．SCE）处产生灵敏的极谱波。应用此极谱波测定HRP的检出限达到3．5×10-12g／mL，线性范围为6．0×10-12～4．0×10-9g／mL。对此酶催化反应产物的电极还原过程也进行了详细的探讨。

A voltammetric enzyme - linked immunoassay based on a newsystem of o - aminophenol (OAP) - H2O2 - horseradish peroxidase (HRP) hasbeen used in the detection of HRP and labelled HRP. By this method, the enzyme- catalyzed reaction of OAP with H2O2 catalyzed by HRP generates anintermediate product o - benzoquinone imine, which produces a sensitivevoltarnmetric peak at - O. 87 V (vs. SCE ) in Britton - Robinson (BR) buffersolution. By using this voltammetric peak, HRP can be measured with a detectionlimit of 3. 5 X 1O- 12 g/mL and a linear range of 6. 0 x l0-12 ~ 4. 0 x 1O -9g/mL. The process of the electroreduction of the product of the enzyme - catalyzedreaction was discussed.