反相色谱法测定洛伐他丁含量的方法

The Determination of Lovastatin Concentration by Reverse-Phase Liquid Chromagraphy

应用反相色谱法对测定片剂中洛伐他丁的含量的方法作了回收率（99．6O％）试验，线性关系相关系数r=0．9953，重现性及稳定性实验一周前后测定量平均百分比98．08％，结果令人满意。用乙腈：甲醇：磷酸二氢钠=5：1：3流动相。通过标准样品扫描，在230．8nm，238．1nm，246nm处有最大吸收。本试验选用了231nm为测定波长，该方法重现性好，定量准确。

Lovastatin is a new reduction enzyme inhibitor which cures the illness of highcholesterol of human beings. In the concentration determination by the reversed phase liquidchromatography the recovery of Lovastatin is 99. 60 %. The standard curve of lovastatin r = 0.9953. The concentation of tablet was determinated by the reversed phase liquid chromatographyin this paper. It was separated rapidly, accurately and sensitively by a mobile phase of acetonitrile: methanol: sodium dihydrogen phosphate = 5: 1: 3. The detection was scanned at 231nm.Lovastatin solution of acetonitrile: acetate (pH = 4 ) = 80: 20 is steady at least in a week.