大鼠心肌梗死后血浆miR-214表达的变化

The changes of plasma miR-214 expression in rats with post acute myocardial infarction in Rat

目的观察大鼠心肌梗死后血浆中miR-214的表达变化。方法 10只雄性SD大鼠,通过前降支动脉结扎法建立大鼠急性心肌梗死模型,模型制备成功后随机分为心肌梗死后14 d组、28 d组,另设对照组,每组5只动物。抽提血浆中的miRNA,应用荧光定量PCR法检测miR-214的表达。结果与假手术组相比,术后14 d组miR-214的表达增加60%[(1.60±0.09)vs.(1.00±0.06),P<0.01],术后28 d组miR-214的表达增加116%[(2.16±0.13)vs.(1.00±0.06),P<0.01]。结论大鼠心肌梗死后随着心肌梗死时间的延长,血浆中miR-214的表达逐渐上调,miR-214可能参与调控了心肌梗死后心室重塑。

Objective To detect the change of expression level of plasma miR-214 in rats with post acute myocardium infarction.Methods Rats underwent left descending coronary ligation or sham surgery.Rats with MI was assigned to two groups（n=5）.Real-time PCR was developed to detect the expression of miR-214 in plasma.Results The expression level of miR-214 in plasma was up-regulated in the 14th and 28th day post MI.Compared to sham group,the expression level of miR-214 increased by 60%（1.60±0.09 vs 1.00±0.06,P〈0.01） in the 14th day and by 116%（2.16±0.13 vs 1.00±0.06,P〈0.01） in the 28th day post MI.Conclusion The expression of plasma miR-214 increases with ventricular remodeling post MI in rat.MiR-214 may be involved in ventricular remodeling post MI.