大花细辛叶提取物对小鼠热板致痛的镇痛作用

Analgesic Effect of Extracts from Leaves of Asarum maximum Hemsl on Mice Induced by Hot Plate

目的:研究大花细辛叶乙醇提取物的镇痛活性及其镇痛活性组分。方法:通过小鼠热板法,研究大花细辛叶的乙醇提取物以及采用系统溶剂法分离得到的5个组分的镇痛活性。结果:大花细辛叶95%乙醇提取物(500 mg/kg)能提高小鼠痛阈值,正丁醇萃取部分(500 mg/kg)能显著提高痛阈值且优于对照组阿司匹林(200 mg/kg)。结论:大花细辛叶的乙醇提取物有镇痛作用,正丁醇萃取部分是镇痛的主要组分。

Objective ： To study analgesic effect of the ethanol extracts from the leaves of Asarum maximum Hemsl on mice and its active fractions. Method： The five fractions were obtained from the extractor, extracted by 95% ethanol from the leaves of Asarum maximum Hemsl by systematic solvent extraction method respectively using petroleum ether, chloroform, acetic ether, n-butanol and water. Mice pain model was induced by hot plate. Result： The extracts of 95% ethanol （500 （mg · kg^-1） ） from the leaves of Asarum maximum Hemsl could increase the threshold of pain on mice induced by hot plate. The effect of the n-butanol fraction （500 （ mg · kg^- 1 ） ） increasing the threshold of pain notably on mice is better than that of Aspirin （200 （mg · kg^- 1 ） ）. Conclusion： The ethanol extracts from the leaves of Asarum maximum Hemsl have analgesic effects, and the n-butanol fraction is the main active fraction.