微流体数字化技术制备基因芯片微阵列

Preparation of genechip microarrays using microfluid digitalization

采用以脉冲为微流动基本形态、脉冲当地惯性力为主动力的微流体数字化技术进行了基因芯片微阵列制备实验。在搭建的基于微流体数字化技术的基因芯片微阵列制备系统上,实验验证了脉冲点样系统参量(收敛角2θ、微喷嘴内径d、电压幅值U和驱动频率f)对样点直径和脉冲点样稳定性的影响规律。以实验规律为依据,提出了制备样点直径约为100μm的中等密度微阵列的实验路线,制备出了样点平均直径为102.2μm、微阵列密度约为4 000spot/cm2的基因芯片微阵列(点样溶液为3×SSC柠檬酸盐缓冲液)。得到的研究结果可为建立高密度基因芯片脉冲点样技术提供实验研究基础。

A preparation experiment of genechip microarrays was carried out by the microfluid digitalization,in which the pulse is basis forms of the microfluidic flows and the microfluidic flows are driven by the pulsed local inertia force in micro channels.Based on the technology,an experimental system to prepare genechip microarrays was built to study the effect the nozzle inside falloff angle 2θ,nozzle inner diameter d,voltage amplitude U,and the driving frequency f on the droplet diameter and pulse potting stability.The experimental scheme for preparing the microarrays was proposed and then the moderate microarray with the droplet average diameter of 102.2 μm and the density of microarray of 4 000 spot/cm2 were manufactured by using 3×SSC citrate buffer as spotting solution.The results in this paper can provide a experimental basis for establishing a high-throughput microarray pulse spotting technology.