一个玉米叶色突变体的遗传分析与基因定位

Genetic Analysis and Gene Mapping of a Leaf Mutant in Maize

在玉米自交系4H8中发现一个叶色突变体,自然条件下,该突变体幼苗叶色呈淡绿色,生长明显缓慢,逐渐萎蔫死亡。利用出现淡绿色幼苗的自交系4H8杂合体以及其与绿色自交系40-6构建的一套分离群体对叶色淡绿色突变体进行了遗传分析,发现4H8的淡绿色基因由1个隐性核基因控制,初步命名为vl1(Virescent leaf 1),并用SSR分子标记将该基因初步定位在第1染色体的长臂上,位于SSR标记umc1323和umc1709之间,与二者的遗传距离分别为2.1 cM和10.3 cM。该研究结果为今后该基因的克隆和功能分析奠定了基础。

Natural and artificial mutants are as valuable resources for functional genomics and molecular breeding.A leaf-color mutant was identified in progency of a maize inbred line 4H8.Under natural conditions,it was virescent and growed slowly at seedings,then wilted to die gradually.A set of segregating populations derived from two inbred lines,the parents 4H8 and 40-6,were used to study the genetic mechanism of virescent leaf in maize.The result demonstrated that the trait of virescent leaf in the inbred line 4H8 was governed by a recessive gene（vl1）,which was mapped on the long arm of chromosome 1 by SSR analysis,the vl1 was flanked by two SSR markers umc1323 and umc1709,with the genetic distances 2.1 cM and 10.3 cM,respectively.The results will be useful for cloning and functional analysis of it.