一株商品肉鸡腺胃H9N2的分离鉴定及HA基因序列分析

Isolation,Identincation and Molecular Characterization of a H9N2 Isolate from a Broiler Flock Glandular Stomach

从30日龄商品肉鸡腺胃分离到一株具有血凝特点的病毒,经HI交叉抑制试验证实为H9N2病毒,命名A/Chicken/Shandong/KD/09,病毒对SPF鸡无致病性,IVPI为0。对其HA基因进行克隆测序,HA基因氨基酸裂解位点的基序为RSSR↓GL,与弱毒基序一致。A/chicken/Shandong/KD/09分离毒株与参考株HA基因的核苷酸之间的同源性为94.5%～100%,氨基酸序列之间的同源性为95.5%～99.8%。与国外A/ck/Korea/ms96/96、A/Parakeet/chiba/1/97和A/turkey/Wisconsin/1/1966 HA基因核苷酸序列同源性为80.9%～91.5%,氨基酸序列同源性为88.1%～91.5%。系统进化树分析表明,该毒株与近年来国内流行的A/chicken/Shandong/2/02禽流感毒株类似。

A newly H9N2 isolate named A/Chicken/Shandong/KD/09 was collected from a broiler flock glanduar stomach,The biological test showed that the isolate A/Chicken/Shandong/KD/09 exhibited the IVPI value with 0,and the isolate shared a lentogenic motif 335RSSR↓GL340 at the HA protein cleavage site,which indicated that A/Chicken/Shandong/KD/09 was lentogenic.The HA gene nucleotides（amino acids） homologies is 94.5%-100%（95.5%-99.8%） between the isolate A/Chicken/Shandong/KD/09 and the China H9N2 isolates.The HA gene nucleotides（amino acids） homologies is 80.9%-91.5%（88.1%-91.5%） between the isolate A/Chicken/Shandong/KD/09 and A/ck/Korea/ms96/96,A/Parakeet/chiba/1/97,A/turkey/Wisconsin/1/1966 H9N2 isolates.Phylogenetic analysis of HA gene showed that the A/Chicken/Shandong/KD/09 was similar to that of the A/chicken/Shandong/2/02.