摘要
以戊二醛法将氨苄青霉素(Ampicillin,Amp)偶联于牛血清白蛋白(BSA)和卵清白蛋白(OVA),合成免疫抗原BSA-Amp和检测抗原OVA-Amp,并进行了鉴定;用BSA-Amp免疫BALB/c小鼠,应用杂交瘤技术建立了4株分泌抗Amp单克隆抗体的杂交瘤细胞株。ELISA和竞争ELISA结果显示,单克隆抗体1G7、2A11、2D10和3F6的腹水抗体效价分别为1∶2.56×107、1∶1.28×107、1∶5.12×106和1∶5.12×106,Amp的半数抑制浓度(IC50)分别为10.22,3.58,4.03,4.95 ng/mL。Amp单克隆抗体与羧苄青霉素(1G7、2A11、2D10和3F6)、青霉素G(1G7和3F6)、阿莫西林(1G7)等青霉素类抗生素存在显著的交叉反应,而与双氯霉素和邻氯霉素未见明显交叉反应。
Ampicillin（Amp） was coupled to carrier proteins of bovine serum albumin（BSA） and ovalbumin（OVA） to synthetize immunogen BSA-Amp and detection antigen OVA-Amp.Four hybridoma cell lines secreting monoclonal antibodies（mAbs） specific for Amp were established by immunizing BALB/c mice with BSA-Amp using hybridoma technology.The antibody titers of mAb ascites（1G7,2A11,2D10 and 3F6） were determined as 1∶2.56×107,1∶1.28×107,1∶5.12×106 and 1∶5.12×106 in ELISA,and their half maximal inhibitory concentration（IC50） of Amp were 10.22,3.58,4.03,4.95 ng/mL in the inhibitive ELISA,respectively.The Amp mAbs showed significant cross-reactivity to the penicillin antibiotics of carbenicillin（1G7,2A11,2D10 and 3F6）,penicillin G（1G7 and 3F6）,and amoxicillin（1G7）,whereas no obvious cross-reactivity to dicloxacillin or cloxacillin was found.
出处
《华北农学报》
CSCD
北大核心
2011年第3期60-63,共4页
Acta Agriculturae Boreali-Sinica
基金
“十一五”国家科技支撑计划(2006BAK02A21)
