两种组胺受体拮抗剂对高致病性猪蓝耳病临床症状的影响

Effects of Two Histamine Receptor Antagonists on Clinical Symptoms of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome(PRRS)

为了解组胺H1、H2受体拮抗剂对高致病性猪蓝耳病临床症状的影响,探讨内源性组胺在患病仔猪发热、呼吸困难、耳尖发绀等典型临床症状中的作用机理。选择20头30日龄的公仔猪,通过人工感染使仔猪患高致病性猪蓝耳病,并随机分为扑尔敏组、西咪替丁组、阳性组、阴性组。每日测量仔猪直肠体温,称量仔猪日采食量,观察仔猪的临床症状变化情况,并于攻毒后第5 d采用RT-PCR方法检测各组仔猪血液中的猪蓝耳病病毒（PRRSV）。结果：攻毒后第5 d,实验组仔猪血液中均检测到PRRSV,攻毒成功;扑尔敏组仔猪体温变化值显著低于阳性组和西咪替丁组（P〈0.05）,日采食量显著高于阳性组和西咪替丁组（P〈0.05）,且临床症状较阳性组和西咪替丁组轻;攻毒后5~9 d西咪替丁组日采食量极显著高于阳性组（P〈0.01）。结果表明：组胺受体拮抗剂能缓解高致病性猪蓝耳病临床症状,且H1受体拮抗剂较H2受体拮抗剂对于缓解临床症状效果明显。

The aim of the study was to investigate the effect of two histamine recePtor antagonists on clinical symPtoms in swine with highly Pathogenic of Porcine ReProductive and ResPiratory Syndrome,in order to discuss the mechanism of action that endogenesis histamine on clinical symPtoms in PRRS Pigs with fever,dysPnea,ear tiPs cyanoPathy.Twenty and 30-day-old male Pigs were randomly divided into ChlorPhenamine galeate group（CM）,Cimetidine grouP（CMT）,Negative grouP,Positive grouP.Checking Piglet temPerature and weighing feed intake everyday,Observing Piglet＇s clinical symPtoms transformation.RT-PCR based DNA amPlification technique for detecting PRRS in Piglet′s blood in 5 days after attacking PRRSV.Results： PRRSV was detected in Piglet′s blood and onteracting toxic substancesv is success,The CM＇s temPerature variable quantity and daily feed intake was lower than that of CMT and Positive grouP（P0.05） and clinical symPtoms is lighter than of CMT and Positive grouP（P0.05）,The CMT＇s daily feed intake significantly higher than Positive grouP（P0.01） in 5~9 d after attacking PRRSV.it is indicate histamine recePtor antagonists can remit clinical symPtoms,the histamine H1 recePtor antagonists is better than H2 recePtor antagonists.