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板栗疫病菌绿色荧光与红色荧光蛋白共定位载体的构建 被引量：2

Construction of Colocalization Vectors of the Green Fluorescence Protein and Red Fluorescence Protein in Cryphonectria parasitica
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摘要 低毒病毒-板栗疫病菌组合是研究病毒与宿主相互作用的一个优秀的模式系统。我们构建了含绿色荧光蛋白基因gfp的载体pCPXHY2GFP与含红色荧光蛋白基因rfp的载体pCPXG418RFP,并用于转化野生型菌株EP155,获得了以潮霉素为筛选标记、表达绿色荧光蛋白的转化株pCPXHY2GFP/EP155和以G418为筛选标记、表达红色荧光蛋白的转化株pCPXG418RFP/EP155。将载体pCPXG418RFP转化pCPXHY2GFP/EP155,获得的转化株能观察到绿色荧光蛋白与红色荧光蛋白共定位的现象。板栗疫病菌绿色荧光与红色荧光共定位载体pCPXHY2GFP与pCPXG418RFP的构建,为深入研究病毒与宿主相互作用的分子机制提供了强有力的研究材料。 Hypovirus-Cryphonectria parasitica system has emerged as an ideal model to study virus-host interactions.The plasmid pCPXHY2GFP with gfp gene and plasmid pCPXG418RFP with rfp gene were constructed and used to transform C.parasitica strain EP155.The resultant transformant pCPXHY2GFP/EP155 which exhibited hygromycin resistance and green fluorescence protein expression,and the transformant pCPXG418RFP/EP155 which exhibited G418 resistance and red fluorescence protein expression were obtained.The green fluorescent protein and red fluorescent protein colocalization phenomenon was observed in the transformants with plasmid pCPXG418RFP and pCPXHY2GFP/EP155.The vector construction of pCPXHY2GFP and pCPXG418RFP provided valuable experimental materials for studying the molecular mechanism of the interaction between hypovirus and its host fungus.
作者 杨峰 施李鸣 范素华 谭俊 谢涛 商巾杰 陈保善
机构地区 广西大学生命科学与技术学院 广西亚热带生物资源保护利用重点实验室 微生物及植物遗传工程教育部重点实验室
出处 《基因组学与应用生物学》 CAS CSCD 北大核心 2011年第3期308-315,共8页 Genomics and Applied Biology
基金 博士点基金项目(20070593001) 广西自然科学基金(2011GXNSFB018027)共同资助
关键词 板栗疫病菌 绿色荧光蛋白 红色荧光蛋白 共定位 载体构建 Cryphonectria parasitica Green fluorescence protein Red fluorescence protein Colocalization Vector construction
分类号 S436.64 [农业科学—农业昆虫与害虫防治]
  • 相关文献

参考文献28

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二级参考文献35

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共引文献8

同被引文献33

  • 1杨坤宇,刘如石,林鉴,张智洪,张军,夏宁邵.红色荧光蛋白在毕赤酵母中的高效表达[J].厦门大学学报（自然科学版）,2005,44(3):410-415. 被引量：2
  • 2贺春萍,邱世文.稻瘟菌T-DNA插入突变体的培养基筛选试验[J].热带农业科学,2005,25(6):24-26. 被引量：2
  • 3蓝秀万,陈敏玫,桂磊,范云燕,甘文剑,陈保善.G418抗性筛选标记在板栗疫病菌中的应用[J].广西农业生物科学,2007,26(B06):7-11. 被引量：8
  • 4王晓鸣,石洁,晋齐鸣,等.玉米病虫害田问手册-病虫害鉴别与抗性鉴定[M].北京:中国农业科学技术出版,2010.
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二级引证文献4

基因组学与应用生物学
2011年 第3期

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