一株苯酚降解菌的筛选、鉴定及其降解特性

Screening,Identification and Degrading Characteristics of A Phenol Degrading Bacterial Strain

本研究采用逐量分批驯化的方法,从造纸废水中分离得到一株能够以苯酚为唯一碳源生长的苯酚降解菌株F5-1。经形态观察、生理生化特性鉴定及16S rDNA序列分析,将该菌株鉴定为克雷伯菌(Klebsiella sp.)。该菌株能够在7h时完全降解初始浓度为100mg/L的苯酚,降解苯酚主要发生在生长对数期;在pH5.0～9.0,NaCl浓度0～80g/L,温度20～40℃范围内,菌株F5-1均可有效降解初始浓度为100～1200mg/L的苯酚;能够耐受的最大苯酚浓度为1500mg/L。本研究结果表明,F5-1菌株对处理环境条件复杂的含酚废水具有潜在的应用前景。

By gradually increasing the concentration of phenol in the culture medium,a phenol-degrading bacterial strain designated as F5-1 was isolated from the wastewater of a paper mill.The organism was able to utilize phenol as a sole carbon source.Based on morphological,physiological and biochemical characterizations and 16S rDNA sequence analysis,F5-1 was identified as a species of Klebsiella.The strain F5-1 could degrade phenol with initial concentration of 100 mg/L completely in 7 h and the phenol degradation was found to occur in log phase.At pH 5.0～9.0,the strain F5-1 could efficiently degrade phenol with initial concentration from 100 mg/L to 1 200 mg/L in the presence of 0～80 g/L of NaCl at temperature range from 20℃ to 40℃.The highest concentration of phenol that the strain can tolerate was 1 500 mg/L.These results indicated that the strain F5-1 may have potential application in treatment of phenol-containing wastewater with complex environmental conditions.