力竭游泳对大鼠肠组织MDA、Free-SH、ATP含量及Na^+-K^+-ATPase活性的影响

Effect of Exhaustive Swimming on the Activity of MDA,Free-SH,ATP and Na^+-K^+-ATPase in Rats Intestinal Tissue

为探讨力竭性运动引起的运动性肠功能紊乱及其氧化应激损伤,将32只SD雄性大鼠随机分为4组:即对照组(C);运动后即刻组(EX);运动后30min组(EX30);运动后60min组(EX60),测定力竭性游泳后不同时相,肠组织匀浆MDA、游离巯基(Free-SH)和ATP含量。结果显示,运动后肠组织MDA含量在运动后30min,60min显著性增加(P<0.01);运动后Free-SH含量在运动后30min(P<0.05)和运动后60min后(P<0.01)显著下降;运动后30min组ATP含量显著下降(P<0.01);运动后60min,Na+-K+-ATPase活性下降。结果提示,力竭运动使肠组织自由基产生增加,自由基使得肠组织中游离巯基氧化,致使ATP含量下降,Na+-K+-ATPase活性下降可能是造成运动性肠功能紊乱的重要因素之一。

In order to investigate the effects of oxidative stress on intestines function and the exercise induced intestines function disorder,32 male SD rats were divided into four groups（that is control group,immediate post-exercise group,30 min post-exercise group and 60 min post-exercise group）.The research measures the contents of MDA、FREE-SH and ATP contents in rats intestine tissues.The result shows that MDA content has significant increase at 30 min and 60 min after exhaustive swimming while FREE-SH contents has remarkable decrease in the 30 min post-exercise group and 60 min post-exercise group.At the same time,ATP content decline significantly in the 30 min post-exercise group.The result suggests that increase of exercise induced free radicals led to FREE-SH of intestine cross link,which resulted in the decline of ATP content.These might be important factors of exercise induced intestine disorder.