蛹虫草菌丝体液体培养工艺的研究

Study on the cultivation technology of Cordyceps militaris mycelium liquid

采用Plankett Burman设计对影响虫草菌丝体生物量的液体培养基组分及培养条件进行了筛选,找到最佳培养条件。以不同培养基得出的人工栽培蛹虫草菌丝体得率为指标对蛹虫草摇瓶发酵工艺条件进行优化。通过响应面分析法和中心组合设计对主要影响因子进行优化,确定蛹虫草发酵最佳工艺参数为:葡萄糖23.34 g/L,玉米浆12.5 mL/L,接种量9%,发酵温度25℃,KH2PO41 g/L和MgSO4.7H2O 1 g/L,VB12 g/L、发酵转速150 r/min,pH值7.0。最佳培养基条件下的验证试验结果为菌丝体生物量15.59 g/L,与模型的预测值15.56 g/L基本一致,表明模型可较好地预测蛹虫草菌丝体的生物量。

Plankett Burman design is applied to choose the optimum cultivation conditions which may influence the cultivation medium of Cordyceps militaris mycelium liquid and cultivation environments.Taking the yield of Cordyceps militaris mycelium with different medium as index,we optimize the cordyceps fermentation conditions.Both the response method and central combination design are used to optimize the technical parameters which are following： Glucose 23.34 g/L,corn plasma 12.5 mL/L,inoculation quantity 9%,fermentation temperature 25 ℃,KH2PO4 1 g/L,MgSO4·7H2O 1 g/L,VB1 2 g/L,fermentation speed 150 r/min,pH 7.0.At the optimal conditions the product of the mycelia biomass is 15.59 g/L matched to the estimated value 15.56 g/L,which show that the estimated model can be used to predict the yield of the Cordyceps militaris mycelium liquid.