Th17细胞在结核性胸膜炎患者外周血及胸水中的表达以及诊断意义

Th-17 cell in the peripheral blood and pleural fluid of tuberculous pleurisy patients

目的探讨Th17细胞在结核性胸膜炎中的作用。方法收集住院收治的初治结核性胸膜炎患者30例和非结核性胸膜炎患者20例,另选择健康体检人员20例为正常对照组。在入院时和治疗后分别抽取外周血和胸水,并使用流式细胞术检测外周血及胸水中Th17细胞比例的变化。结果我们发现在Th17细胞在结核性胸膜炎患者中出现了显著性的上升,而非结核性胸膜炎患者虽然也出现了一定量的升高,但和结核性胸膜炎患者相比,还是存在显著性的差异。此外,我们又检测了经过治疗的结核性胸膜炎患者的Th17细胞比例,发现经过治疗之后,不论外周血还是胸水中,Th17细胞的比例均有显著性的下降。结论上述结果说明Th17细胞可能全程参与到了结核性胸膜炎的发病过程中,并且可能成为其诊断的重要依据。

Objective to investigate the role of Th-17 cell in the tuberculous pleurisy patients.Methods we collected 30 primary tuberculous pleurisy patients,20 other other pleurisy patients and 20 healthy people as control.We collect their peripheral blood and pleural fluid for T cell culture,and use flow cytometry to identify ratio of Th17 and all T cell.Results we found Th17 would increase in the peripheral blood and pleural fluid of tuberculous pleurisy patients compared with non-tuberculous pleurisy patients and healthy people.And we further found after treatment,the Th-17 cell also decreased both in peripheral blood and pleural fluid.Conclusion our results suggested that Th-17 cell had its specific role in the tuberculous pleurisy,and had the potential to become a further diagnostic marker.