原发性干燥综合征患者外周血Th17细胞的检测及意义

Expression and significance of Th17 cell from peripheral blood of patients with primary sjogren's syndrome

目的探讨Th17细胞原发性干燥综合征(pSS)患者外周血中表达水平及意义。方法分离pSS患者和健康者外周血单个核细胞(PBMC),磁珠阴选CD4+T细胞,然后加佛波酯/离子霉素,经过固定及透膜处理后进行细胞内染色,流式细胞术(FCM)检测CD4+T细胞内白细胞介素17(IL-17)水平。结果免疫磁珠阴选CD4+T细胞纯度>90%。pSS患者Th17细胞水平(2.14±0.91)显著高于正常对照组(0.56±0.21),P<0.01;pSS患者TH17细胞水平与ANA滴度、Anti-SSA、Anti-SSB、C3、C4无显著相关性(P>0.05)。结论 pSS患者外周血中TH17细胞表达增高,Th17细胞表达异常增高可能是pSS发病的重要环节。

Objective To investigate the expression and significance of Th 17 cells from peripheral blood of patients with sjogren＇s syndrome（pSS）.Methods Intracelluar flow cytometry detection of IL-17 was established using PMA as stimulators after isolution of untouched human CD4＋ T cells from PBMC.Results The isolation of untouched human CD4＋ T cells from PBMC was effective and its purity was over 90%.The percentage of intracelluar IL-l7 in CD4＋ T cells from pSS patients was increased significantly higher.Such percentage in pSS（2.14±0.91） was higher than that of healthy controls（0.56±0.21）,P0.01.There was no correlation between Th 17 cells and serum concentration of Anti-SSA,Anti-SSA,ANA,C3 and C4 in pSS patients（P0.05）.Conclusion There is an abnormal expression of Th 17 cells in pSS patients.These data indicated that Th 17 cells might play certain role in the pathogenesis and progress in pSS.