骨肽注射液中磷含量的测定

Determination of Phosphorus in Ossotide Injection by Molybdenum Blue Spectrophotometry

目的：建立了骨肽注射液中磷含量的测定方法,并进行了方法学验证。方法：该方法采用钼蓝分光光度法,将样品用硫酸、高氯酸及硝酸消化,冷却后,加入显色试剂,用紫外可见分光光度计在波长820 nm处测定吸收值。结果：线性范围为0~34μg;相关系数R为0.9993;平均回收率为97.8%。结论：采用本法检测骨肽注射液中磷的含量,方法灵敏、准确、可靠。

Objective：The method for determination of phosphorus in Ossotide Injection was established,and the verification methodology was carried out.Methods：The method adopts molybdenum blue spectrophotometry to firstly digest the sample with sulfuric acid,perchloric acid and nitric acid,then after cooling,add the color reagent,and finally,measure the absorbance at a wavelength of 820 nm by UV/Vis spectrophotometer.Results：The linear range of phosphorus was 0～34μg,（R=0.9993）;The average recovery was 97.8%.Conclusion：This method was found to be sensitive and accurate and useful in control the content of phosphorus in Ossotide Injection.