RP-HPLC法测定精制冠心颗粒中芍药苷含量

RP-HPLC Determination of Paeoniflorin in Jingzhiguanxin Granules

目的:建立RP-HPLC法精制冠心颗粒中芍药苷的含量。方法:hypersil NH2色谱柱(5μm 4.6mm×150mm));甲醇-水(25:75)为流动相;流速为1.0mL.min-1;紫外检测波长为230nm。结果:芍药苷线性范围为1.006μg^10.06μg,回归方程:Y=6715203.21X+25035.10(r=0.999 8),平均回收率为98.68%,RSD=0.70%。结论:该方法简便、灵敏、准确,重复性好,可用于精制冠心颗粒的质量控制。

Objective：To establish a RP-HPLC method for the determination of Paeoniflorin in Jingzhiguanxin Granules.Methods：Using Phypersil NH2 column（5μm,4.6mm×150mm）;mobile phase was methanol-water（25：75）;flow rate was 1.0ml·min-1;detective wavelength was 230nm.Results：The liner range of the detemaination is 1.006-10.06μg,（r = 0.999 8）,the average recovery was 98.68 %,RSD%=0.70%.Conclusion：The method is simple,sensitive,accurate,reproducible and can be used for quality control of Jingzhiguanxin Granules.