VEGF、P53、bcl-2在胃癌组织中的表达及临床意义

目的研究癌基因VEGF、P53、bcl-2在胃癌组织中的表达及其与肿瘤标志物CEA、CA199的相关性。方法收集45例在本院手术的胃癌患者标本，采用SP法检测VEGF、P53、bcl-2在胃癌组织中的表达，并同时抽取静脉血作CEA、CA1992种肿瘤标志物的测定。结果P53的表达与胃癌侵犯深度相关（X^2=9．17，P〈0．01），P53/、VEGF在发生远处淋巴结转移的阳性表达率均为95％，明显高于未发生转移者的64．3％和50．O％，bcl-2与淋巴结转移无相关性，与浸润深度有关（在肌层高表达）。CEA、CA199与远处淋巴结转移及生存期相关，阳性率高提示易发生远处转移，且生存期〈1年，同时发现，R，与CEA、CA199呈正相关、与VEGF呈正相关、与bcl-2呈负相关。结论胃癌手术前联合检测R，、VEGF、bcl-2可以对疾病的分期、浸润深度、淋巴结转移作一个粗略的评估，同时测定血清CEA、CA199能对患者的预后作出预测，以便安排术后足疗程的化疗、免疫治疗，以避免术后胃癌早期复发，延长患者生存期。

Objective To study the expression of VEGF P53 bcl -2 in gastric carcinoma and their relation with CEA CA199. Method 45 cases gastric carcinoma＇s biopsy were collected after operation in our hospital, we measured VEGF P53 bcl - 2 in biopsy with SP, and measure CEA CA199 with patients＂ blood serum. Result Expressoin of P53 had significant relation to gastric carcinoma＇s soakage depth （ X^2 = 9. 17, P 〈 O. 01 ）, positive ratio of P53 VEGF was higher in cases with beyond lymph node＇s transfusion than those withnot（64.3% vs 50.0% ）, bcl- 2 had no relation with lymph node transfusion, but had relation with soakage depth, it had higher expression in muscle layer. CEA and CA199 had relation to lymph node＇s beyond transfusion and sm＇vival period, cases with higher positive ratio had short survival period, shorter than one year. And we found P53 had positive relation to CEA CA199,negative relation to bcl -2, but had correlation to VEGF. Conclusion To measure P53 bcl -2 and VEGF before gastric carcinoma＇s operation, and measure CEA CA199 in the meantime, we could get a rough evaluation on the disease＂ stage, soakage depth and lymph node transfusion, then we could arrange plenty chemotherapy period, immunity treatment, so as to refrain from disease＇relapse in early stage, and prolong patient＇s survival period.