中国樱桃泰小红樱的组培快繁研究

Tissue Culture of Chinese Cherry Cultivar Taixiaohongying

以中国樱桃'泰小红樱'的茎段为外植体,研究WPM和MS不同培养基以及6-BA和NAA不同激素浓度组合对外植体增殖的影响,建立其组织培养的快繁体系。结果表明:(1)泰小红樱茎段最佳的初代培养基为MS+1.0 mg/L6-BA+0.1 mg/L NAA,诱导分化率高,腋芽新梢生长健壮;(2)最佳继代培养基为WPM+1.0 mg/L6-BA+0.1 mg/L NAA,丛芽增殖与腋芽增殖并存,增殖倍数显著提高,高达8.0倍,且芽苗健壮,叶色浓绿;(3)最佳生根培养基为1/2MS+0.5 mg/L IBA+20 g/L蔗糖+5 g/L琼脂,生根率高,根系长且数量多。

The shoots of Chinese cherry cultivar Taixiaohongying were used as explants and cultured on WPM and MS media with different concentrations of 6-BA and NAA.The culture effects were studied and the results were as follows.（1） The best culture medium for shoots was MS＋1.0 mg/L 6-BA＋0.1 mg/L NAA.On this medium,the axillary buds sprouted easily and grew vigorously.（2） The best multiplication culture medium was WPM＋1.0 mg/L 6-BA＋0.1 mg/L NAA.On this medium,the multiplication of axillary buds and cluster buds coexisted,so the regeneration multiple increased significantly and reached eight times;the regenerated shoots grew vigorously and had dark green leaves.（3） The best rooting medium was 1/2MS＋0.5 mg/L IBA＋20 g/L sucrose＋5 g/L agar.On this medium,the rooting rate was higher,and the roots were longer and more.