转BADH和Bar基因水稻培育及其相关特性评价

Breeding and Traits Evaluation of BADH and Bar Gene Transgenic Rice

采用基因枪法将目的基因AhBADH和Bar转入水稻不育系P88S基因组中,经过PCR和Southern杂交检测,发现目的基因已整合到水稻基因组中.草铵膦发芽试验说明Bar基因已经在植株中正常表达.耐盐鉴定试验表明,转基因株系较对照耐盐性明显提高.光温敏核不育系P88S经过转基因后育性发生改变,表现出一定的可育度.

The AhBADH gene（encoding betaine aldehyde dehydrogenase） and Bar gene were transferred into genome of rice variety P88S by particle bombardment,and the integrations in rice genome were con-firmed by PCR and Southern blotting.Germination in herbicide glufosinate showed that Bar gene was ex-pressed in rice plants.Salt stress demonstrated that the salt tolerance of transgenic line was increased over non-transgenic line.The pollen sterility of PTGMS（photoperiod-sensitive / thermo-sensitive genic male sterile） line P88S changed after genetically modified and showed some degree of fertility.