基于线粒体Cytb基因序列探讨中国斑翅蝗科部分种类的系统发育关系

Phylogenetic Relationships of the 14 Oedipodidae Species (Insecta:Orthoptera) Based on the Cytb Gene Sequences

基于斑翅蝗科14种的线粒体Cytb基因462 bp序列,使用MEGA2和PAUP*4.0b软件包进行分析,显示Cytb基因序列具有明显的高A、T偏向性和距离依赖的TS/TV值.采用邻接法(NJ)、最大似然法(MP)和极似然法(ML)分别构建斑翅蝗科4亚科8属的系统发育树,不同算法构建的系统发育树均支持分为四大分支(亚科):A(绿纹蝗属)、B(束颈蝗属)、C((飞蝗属+车蝗属)+小车蝗属)、D((痂蝗属+异痂蝗属)+皱膝蝗属);红胫小车蝗和黄胫小车蝗作为两个种比较合适;痂蝗亚科和异痂蝗亚科应该合并为一个亚科;飞蝗亚科与斑翅蝗亚科的分类关系尚未解决,需要进一步的研究进行分析验证.

The mitochondrial Cytb gene of 14 Oedipodidae species were sequenced.The MP,ML and NJ phylogenetic trees of these species were reconstructed by using the MEGA2 and PAUP＊4.0 softwares.The 462 bp of Cytb gene showed typical high A＋T content and variable distance-dependent transition/transversion ratios.All these trees produced essentially the same results as follows： The Oedipodidae family can be di-vided to four subfamilies.A（Aiolopus）,B（Sphingonotus）,C（（Locusta ＋ Gastrimargus） ＋ Oedaleus）,D（（Bryodemella ＋ Bryodema） ＋ Angaracris）;It is appropriate to consider the Oedaleus manjius and the Oedaleus infernalis as two separate species;The two subfamilies of the Bryodemellinae and Bryodeminae are closely related and they should be assigneded as one subfamily;The phylogenetic stata of the Oedipodi-nae and Locustinae are unresolved in this study and should be further investigated.