黑线仓鼠4.5S RNA cDNA的克隆及序列分析

Cloning and Sequence Analysis of 4.5S RNA in Cricetulus Barabensis

4.5S RNA是一种特异存在于啮齿类细胞中丰富的核小RNA分子(small nuclear RNA,snRNA).研究以黑线仓鼠(Cricetulus barabensis)为材料,从新鲜肝脏中提取总RNA,以此反转录4.5S RNA cDNA序列,并克隆测序,所得核苷酸序列为88 bp(Genbank登录号:HM146085).将该序列与啮齿动物中其他物种的4.5SRNA序列进行同源性分析,系统进化分析结果与物种亲缘关系的远近基本一致,表明该研究所得到的4.5SRNA基因序列可作为研究物种亲缘关系或遗传距离的标记.此结果为进一步研究黑线仓鼠4.5S RNA的分子结构及其生物学功能奠定了基础.

4.5S RNA is a rodent-specific small noncoding nuclear RNA.In this paper,total RNA was extracted from the liver tissue of Cricetulus barabensis,the cDNA of 4.5S RNA was amplified by reverse transcription-polymerase chain reaction method,then we constructed the vector and sequenced.The nucleotide sequence of this gene is 88 bp and its accession number in Genbank is HM146085.The result of the the phylogenetic evolution analysis by 4.5S RNA of different species is similar to the relative species relationship,and therefore the 4.5S RNA coulde be used as an ideal marker to study the species relationships or hereditary distance.Meanwhile the result is also used for the further study of the molecular structure and laid the foundation for biological function of 4.5S RNA in Cricetulus barabensis.