离子对色谱法同时测定水中多种氯代酸性除草剂

Simultaneous Determination of Chlorinated Acidic Herbicides in Waters by Ion-pair Chromatography

建立了离子对色谱法同时测定水中DCPA二元酸、4-硝基苯酚、麦草畏、苯达松、2,4-二氯苯乙酸、2-甲基-4-氯苯氧乙酸（MCPA）、2-（4-氯苯氧基-2甲基）丙酸（MCPP）、3,5-二氯苯甲酸、2,4-滴丙酸9种氯代酸性除草剂。通过加入离子对试剂使多种极性强、保留弱的酸性除草剂在普通的反相色谱柱上同时分离。采用Shim-pack VP-ODS色谱柱,流动相为V（1-辛烷三乙磷酸铵溶液）∶V（乙腈）∶V（甲醇）=6∶3∶1,流速1.8 mL/min,不同组分全流程方法检出限范围为0.03-0.15μg/L,相对标准偏差在10%以下。建立的方法可将9种除草剂完全分离,与高效液相色谱法和气相色谱-质谱法相比,具有操作简单、分离效果好、回收率高、干扰少的优点,能够满足不同水质的检测要求。

A method for the simultaneous detection of several chlorinated acid herbicides such as dicamba and bentazon etc by ion-pair chromatography was developed.Several strongly polar and weak reserve chlorinated acid herbicides can be separated simultaneously from reversed-phase chromatography column by adding ion-pair reagent.Shim-pack VP-ODS column was used for analyte separation with ion-pair solution and the mobile phase was at a constant flow rate of 1.8 mL/min.Extraction condition,ion-pair and the mobile phase were selected and optimized.The detection limit ranges of the method for different herbicides were 0.03 to 0.15 μg/L with RSD less than 10%.Comparing with high performance liquid chromatography and gas chromatography-mass spectrometry,the established method completely separates the 9 sorts of herbicides with advantages of simple operation,high separating effect,high recoveries and less interferences,which meet the requirements of all kind of water.