Cd、Pb与酸雨复合污染对油菜种子萌发和幼苗保护酶活性的影响

Effect of Cd and Pb and Simulated Acid Rain on Emergence and Activity of Cell Defense Enzymes of Rape seedlings

采用沙基培养方法,研究不同浓度的Pb、Cd和pH5.2的酸雨混合液对其种子萌发、幼苗叶片叶绿素含量、幼苗保护酶活性等的影响。结果表明:Pb、Cd溶液浓度在100 mg/L以内,随胁迫程度的增加,幼苗总重、全长、根重出现了下降。POD(过氧化物酶)、SOD(过氧化物歧化酶)活性先增加后下降,但都较对照组高,CAT(过氧化氢酶)活性一直增强。种子发芽率随重金属浓度的增加表现为低促进高抑制的现象,叶绿素a、b含量在5 mg/L浓度内呈上升趋势,大于5 mg/L时出现了减少,总体上呈波动变化。

Using rape as materials,effect of simulated acid rain of pH5.2 and six different levels of Pb,Cd on the activity of cell defense enzymes system,leaf chlorophyll content,germination rate was studied.The results showed that when Pb and Cd was less than 100mg/L,total length of seedlings,weight of seedlngs and weight of roots were decreased as stress increased.The activities of SOD,POD were increased at first and decreased later.However,their activities were higher than that of control.Activity of CAT was increased all time.Germination rate was increased when heavy metal elements were low,but decreased when heavy metal elements were higher.When the levels of Pb and Cd was less than 5mg/L,leaf chlorophyll content was increased,but it was decreased when the levels of Pb and Cd was more than 5mg/L.Chlorophyll a/b was also increased at first and decreased later.