RT-PCR法克隆柑橘皮果胶酯酶基因

Gene Cloning of PME from Citrus Peels by RT-PCR

试验利用RT-PCR技术将新鲜柑橘皮果胶酯酶基因克隆到pGEM T-Easy载体上,并进行了鉴定和序列测定。结果表明:该基因为克隆成功的果胶酯酶基因,全长1797bp,开放阅读框架长1785bp,共编码594个氨基酸。该序列含有PMEI和Pectinseterase两个保守序列。该序列与Covadonga R发表的citrus CsPME4 3′端序列氨基酸同源性100%。

The PME gene was amplified from citrus peels by RT-PCR technique and inserted into plasmid pGEM T-Easy.The interest fragment was identified and sequenced.The results showed that it presented 100% identity with the 3′extremitycitrus of CsPME4.It has 1797bp and its ORF is 1785bp which coding 594 amino acid.It has two conserved sequences： PMEI and Pectinseterase.These suggest that is PME gene from citrus peels.