参麦注射液对淋巴管内皮细胞增殖及凋亡的影响

Effects of Shenmai Injection on Proliferation and Apoptosis of Lymphatic Endothelial Cells

目的探讨参麦注射液对淋巴管内皮细胞（lymphatic endothelial cells,LECs）增殖和凋亡的影响。方法取健康猪的胸导管内皮细胞进行分离、培养;采用Ⅷ因子、血管内皮生长因子受体3（vascular endothelial growth factor receptor-3,VEGFR-3）抗体联合对LECs进行鉴定;采用甲基噻唑基四唑（methyl thia-zolyl tetrazolium,MTT）染色法检测参麦注射液对LECs的生长抑制作用;采用Hoechst 33258荧光染色法检测LECs凋亡。结果形成了典型的LECs;当浓度为40~160μg/ml时,参麦注射液能明显抑制LECs的增殖,且抑制作用呈现剂量依赖关系;Hoechst 33258荧光染色检测证实,经参麦注射液处理后LECs,可观察到其核周围有凋亡小体。结论参麦注射液能抑制淋巴管生成和诱导LECs凋亡。

Objective To explore the influence of Shenmai Injection,a patent traditional Chinese medicine mainly composed of Ginseng,and Radix Ophiopogonis,on the proliferation and apoptosis of lymphatic endothelial cells（LECs）.Methods LECs were isolated and cultured from healthy porcine thoracic duct,and they were identified with Ⅷ factor and vascular endothelial growth factor receptor-3（VEGFR-3）.The inhibitory effect of Shenmai Injection on the growth of LECs was observed by methyl thiazolyl tetrazolium assay,and the apoptosis of LECs was detected by Hoechst33258 fluorescent staining.Results LECs were found to be typical.Shenmai Injection could significantly inhibit the proliferation of LECs within the concentration of 40 to 160 μg/ml,and the inhibitive effect showed a concentration-dependent manner.Hoechst 33258 fluorescent staining showed some apoptotic bodies around the nuclear of LECs treated with Shenmai injection.Conclusion Shenmai Injection can inhibit lymphangiogenesis,and induce the apoptosis of LECs.