规模化猪场猪霍乱沙门氏菌的分离鉴定与药敏试验

Identification and Drug Sensitivity Test of Salmonella choleraesuis Isolated from the Scale Pig Farm

采集新乡市某规模化猪场疑似猪沙门氏菌病的病料,通过细菌分离培养、生化鉴定、动物试验和药敏试验,分离到1株革兰氏阴性小杆菌,该菌在麦康凯琼脂平板上呈细小、无色半透明、光滑、圆整的菌落,对葡萄糖、木糖醇、赖氨酸、甘露醇、蔗糖、果糖和山梨醇反应阳性,对甘露糖、硫化氢、苯丙氨酸、尿素酶反应呈阴性,可致死小白鼠,确定所分离的细菌为猪霍乱沙门氏菌。该菌对头孢噻呋、左旋氧氟沙星等敏感,对诺氟沙星和新霉素中介,对氟苯尼考、氨苄西林、庆大霉素等耐药。该研究为猪沙门氏菌病的防治提供理论依据。

Some organic and tissue samples of the diseased pigs of suspected swine salmonellosis were collected from a scale pig farm in Xinxiang city.Bacterial isolating culture,biochemical identification,animal experiments and drug sensitivity were performed.The results showed that one small Gram-negative bacillus which represented the needle-shape,clour-less transparent,smooth and circular on the Macconkey medium was isolated.Positive reactions to glucose,xylitol,lysine,mannitol,sugar,fruit sugar and sorbitol rather than mannose,hyduogen sulphide,phenyla lanine,urease were observed.The bacillus can cause death in little white mouse.This indicates that the bacteria presently isolated were Salmonella choleraesuis.The bacterium was sensitive to ceftiofur sodium and levofloxacin,intermediate to norfloxacin and neomycin,but resistant to florfenicol,ampicillin and gentamicin.The data provided some theoretic guidance for the prevention and treatment of swine salmonellsis.