星点设计-效应面法优化纤维素酶提取黄精多糖

Optimal Extraction of Polysaccharides Polygonatum by Cellulase with Central Composite Design-Response Surface Methodology

采用星点设计-效应面优化法探讨纤维素酶提取黄精粗多糖的最适宜工艺条件。在单因素试验基础上选取酶解温度、酶解pH值和加水量为自变量,黄精粗多糖提取率为因变量,对自变量各水平进行二项式拟合,用效应面法选择较适宜工艺条件,并进行预测分析。结果表明,黄精粗多糖最适宜提取工艺为:纤维素酶用量为黄精质量的0.50%,酶解时间120 min,酶解温度49℃,酶解pH值4.6,加水量7.2倍,二项式拟合复相关系数R为0.957 4。在此优化条件下提取的黄精多糖提取率为(16.3±0.5)%。星点设计-效应面法建立的模型可较好地描述该试验中因素与指标的关系,预测性良好。

Based on central composite design and response surface methodology,the optimal extraction of Polygonatum polysaccharides by cellulase was investigated.Experiment factors and levels were firstly selected by one factor tests.Independent variables were the enzymolysis temperature,the pH of extracting media and the ratio of liquid,and dependent variable was extraction rate of polysaccharides in Polygonatum.Nolinear mathematic models were used to analysis the relationship between independent and dependent variables.Response surface methodology was used to optimize the process of extraction.Regression coefficients of binomial fitting complex model was 0.957 4,under the optimal extraction process of enzymolysis with 0.50% enzyme concentration for 120 min under 49 ℃,and 7.2 times the amount of water at pH 4.6.The yield of crude polysaccharides of Polygonatum extracted by cellulase was（16.3±0.5）% under the optimum conditions.The established model can describe the relationship between factors and index,and the outcome of prediction is good.