不同临床类型HBV感染患者肝组织中单核细胞趋化蛋白-1的表达及意义

Expression and significance of MCP-1 in liver tissue of different clinical types of HBV infected patients

目的:研究乙肝病毒(HBV)携带者、慢性乙型肝炎(CHB)、乙型肝炎肝硬化患者肝组织中单核细胞趋化蛋白-1(MCP-1)的表达,并探讨其临床病理意义。方法:20例HBV携带者、18例CHB患者、13例乙型肝炎肝硬化患者肝组织经10%中性甲醛固定后制作石蜡包埋切片,并以两份正常人肝组织作为对照,采用免疫组织化学法(SP法)检测肝组织MCP-1的表达水平。结果:正常肝组织MCP-1无表达,HBV携带者、CHB、乙型肝炎肝硬化患者肝组织MCP-1表达阳性率依次为25%、77.78%、84.62%,差异均有显著性意义。结论:MCP-1在肝组织中的表达随肝脏炎症和纤维化的发展而升高,MCP-1可能在病毒性肝炎肝脏炎症和纤维化的病理过程中起重要作用。MCP-1的研究对乙型病毒性肝炎的病情诊断、发病机制探讨及治疗提供新思路。

Objective：To study the expression of monocyte chemoattractant protein-1（MCP-1） in the liver tissue of hepatitis B virus carriers,chronic hepatitis B,hepatitis B liver cirrhosis patient and its clinical pathological significance.Methods：Twenty cases of hepatitis B virus carriers,18 chronic hepatitis B patients,13 patients with liver cirrhosis were studied.Their liver tissue were fixed in 10% neutral formalin and paraffin-embedded.2 cases of normal liver tissue was as control team.The expression of MCP-1 in liver tissue were detected by Immunohistochemical method（SP）.Results：No expression of MCP-1 In normal liver tissue.Positive expression of MCP-1 rates were 25%,77.78%,84.62% in hepatitis B virus carriers,chronic hepatitis B,hepatitis B liver cirrhosis patients respectively.The difference were significantly.Conclusion：The expression of MCP-1 in liver tissue increase with the development of liver inflammation and fibrosis.MCP-1 may play an important role in pathological processes of liver inflammation and fibrosis.The study of MCP-1 maybe give a new ideas in the diagnosis,pathogenesis and treatment.