秦岭太白米中黄酮类成分的研究

Conponent Analysis of Flavonoids from Notholirion hyacinthinum Srom Qinling Mountains

将太白米鳞茎及组织培养诱导的愈伤组织、腋芽和不定芽的提取物进行紫外可见光光谱分析和高效液相色谱分析。结果表明,通过组织培养获得的愈伤组织、腋芽和不定芽中总黄酮的含量分别为12.6、8.29、.2 mg.g-1,其中愈伤组织中的含量与鳞茎12.9 mg.g-1无显著差异,为愈伤组织代替太白米鳞茎入药提供了一定的依据;太白米的鳞茎及愈伤组织、腋芽、不定芽中均不含有芦丁、槲皮素和儿茶素,含有黄岑素;提取物中黄岑素的含量差别明显,愈伤组织、腋芽、不定芽中黄岑素的含量基本相同,但约为鳞茎的1/2。

Contents of flavonoids contained in the extracts of callus,axillary and adventitious buds of Notholirion hyacinthinum were measured by UV-visible spectral analysis and HPLC analysis as 12.6 mg·g-1,8.2 mg·g-1,9.2 mg·g-1,respectively.No significant differences were found between the contents of flavonoids in callus（12.6） and bulbs（12.9）,indicating that the callus may substitute for bulbs for officinal.Baicalein was found both in bulbs and callus,axillary and adventitious buds.Rutin,quercetin and catechin did not detected in both.Contents of baicalein in callus,axillary and adventitious buds were similar,about half of that in bulbs.