IL-12联合雷帕霉素增强PRRS弱毒疫苗诱导的细胞免疫

IL-12 combined with Rapamycin enhances the ellular immunity induced by PRRS attenuated vaccine

将白介素-12(Interleukin-12,IL-12)联合雷帕霉素(Rapamycin,RAPA)作为免疫佐剂,与猪繁殖与呼吸综合征(Porcinereproductive and respiratory syndrome,PRRS)弱毒疫苗联合免疫小鼠后,评价其诱导产生的细胞免疫水平,探讨将IL-12和雷帕霉素作为PRRS弱毒疫苗免疫佐剂的可行性。首先将PRRS弱毒疫苗免疫小鼠,然后连续3 d注射(包括免疫当天)IL-12和不同剂量的雷帕霉素,通过ELISA试剂盒检测小鼠血清中IFN-γ的含量,MTT法检测小鼠脾淋巴细胞的增殖,通过流式细胞仪检测记忆性CD8+T细胞比例的变化。结果显示,将IL-12联合低剂量雷帕霉素作为PRRS弱毒疫苗免疫佐剂可明显促进小鼠脾淋巴细胞的增殖,IFN-γ的分泌,,提高记忆性CD8+T细胞的数量。说明IL-12联合低剂量雷帕霉素可增强PRRS弱毒疫苗诱导的细胞免疫应答,具有作为免疫佐剂的可行性。

To investigate the possibility of the interleukin-12 combining with Rapamycin as adjuvant of PRRS attenuated vaccine,the paper assessed the cellular immunity of mice immunized with PRRS attenuated vaccine.Firstly,the mice were immunized with PRRS attenuated vaccine,then injected with IL-12 and Rapamycin three times one a day（including the immunization day）.Levels of IFN-γ in mice serum,spleen lymphocyte proliferation and memory CD8＋T cell ratio were detected respectively.The results showed that IL-12 combining with low dose of Rapamycin could promote the proliferation of T lymphocytes significantly,boost the secretion of IFN-γ and increase the number of memory CD8＋T cells.It indicated that IL-12 combining with low dose of Rapamycin could enhance the cellular immune response induced by PRRS attenuated vaccine,and could be used as adjuvant.