猪源益生菌株Bacillus velezensis Z-27产芽孢发酵条件优化

Optimization of spore producing fermentation conditions of probiotic strain bacillus velezensis Z-27 from pig

对猪源益生菌株Bacillus velezensis Z-27进行发酵条件优化试验。以芽孢产率和生物量为指标,利用单因素试验和正交试验确定了摇瓶发酵的最佳条件:玉米粉1%、豆饼粉1.5%、MnSO40.03%、NaH2PO4.2H2O 0.2%、Na2HPO4.2H2O 0.4%,初始pH值7.0,250 ml三角瓶装量50 ml,接种量2%,摇床转速220 r/min,发酵温度37℃。优化后发酵液的生物量为2.33×1010 cfu/ml,芽孢产率达96.0%,为该菌株的进一步研究和应用奠定了基础。

Spore producing fermentation conditions of probiotic strain bacillus velezensis Z-27 from pig were optimized.With spore yield and biomass as main indicators,single factor test and orthogonal test were done to determine the suitable conditions for fermentation in shake flask.Results showed that biomass was 2.33×1010 cfu/ml and spore yield was 96.0% at the optimal condition that corn meal was 1%,soybean meal 1.5%,MnSO4 0.03%,NaH2PO4·2H2O 0.2%,Na2HPO4·2H2O 0.4%,initial pH 7.0,media volume 50 ml/250 ml,inoculation volume 2%,rotation speed 220 r/min,cultured for 24 h at 37 ℃.It laid foundation for further research and application of strain bacillus velezensis Z-27.