摘要
为了测定41种抗衰老中草药的抗氧化活性,筛选了抗氧化作用强的中草药。实验采用70%的乙醇作溶剂,超声细胞破碎仪对药物进行提取,抗氧化能力的测定使用分光光度计,应用DPPH和FRAP进行测定。通过测定41种中草药乙醇提取液对DPPH自由基的清除能力和FRAP值,表征其抗氧化能力。结果表明:何首乌、大黄、淫羊藿、山楂、白芍、山茱萸等在10 mg/mL的浓度下,对DPPH自由基的清除率超过70%,总抗氧化能力的FRAP值>200,抗氧化能力较强;其他药物也有不同程度的抗氧化活性,但作用较弱。
The antioxidative activities of 41 kinds of Chinese herbal medicines were determined and selected.The antioxidative substances were extracted from Chinese herbal medicines by 70 percent ethanol and omni ruptor.The spectrophotometric method was used to study the antioxidant activities of medicinal herbs by DPPH and FRAP methods.The results show that these kinds of medicinal herbs including Polygonum multiflorum,Rheum pamatum,Epimedium grandiforum,Crataegus tifiga,Paeonia lactiflora,Cornus officinalis have strong antioxidant activities.When the concentration is 10 mg/mL,the extracts from these six herbs reduce at least 70% of DPPH and the FRAP value of antioxidative capacity is more than 200,and the others have weaker antioxidative activity.
出处
《实验室研究与探索》
CAS
北大核心
2011年第6期11-14,共4页
Research and Exploration In Laboratory
基金
"十一五"国家科技支撑计划资助项目(2006BAK02A03-3)
河南省青年骨干教师资助计划
关键词
分光光度法
中草药
FRAP法
DPPH法
抗氧化活性
spectrophotometry
Chinese herbal medicines
DPPH method
FRAP method
antioxidative activity
