摘要
从40份农田土壤中分离筛选出了69株芽孢杆菌对Fusarium oxysporum、Botrytis cinerea、Colletotrichum gloeosporioides、Cylin-drocarpon具有拮抗作用,其中Bs2菌株发酵液稀释50倍(5.6×107cfu/mL)后处理,连续用3次后的第7天,对辣椒白粉病的防治效果达到60.77%,略低于化学农药粉锈灵(0.8 g/L)的防治效果。通过形态特征观察,生理生化试验及16S rDNA序列分析,Bs2与菌株Bacillus pumilusSBT2-9的相同,其16S rDNA序列同源性高达99.41%,被鉴定为短小芽孢杆菌。
69 strains antagonistic against Fusarium oxysporum,Botrytis cinerea,Colletotrichum gloeosporioides,Cylindrocarpon,were screened from the 40 field soil samples.A strain Bs2 with 50-fold dilution(5.6×107cfu/mL) of the fermentation broth gave good control efficacy of 60.77 % against hot pepper powdery mildew in the field test after 3 sprays,which was lower than that of fungicide triadimefon(0.8 g/L).Bs2 was identified as Bacillus pumilus on the basis of its morphological,physiological and biochemical characters as well as its sequence analysis of 16S rDNA with 99.41 % similarity to B.pumilus SBT2-9.
出处
《西南农业学报》
CSCD
北大核心
2011年第3期974-978,共5页
Southwest China Journal of Agricultural Sciences
基金
科技部国际科技合作项目"防治植物病害和促进植物生长的微生物开发应用及机制研究"(2009DFA32360)
关键词
辣椒白粉病
拮抗细菌
鉴定
短小芽孢杆菌
生物防治
Hot pepper powdery mildew
Antagonistic bacterium
Identification
Bacillus pumilus
Biological control