摘要
以青檀(Pteroceltis tatarinowii Maxim.)的叶片为材料,采用改良高盐低pH值法、改良SDS法、改良CTAB法和试剂盒法4种不同方法提取其基因组DNA,并进行电泳分析、质量检测及ISSR引物扩增检测.结果表明,改良CTAB法优于其它方法,提取的DNA质量较好,纯度较高,能满足进一步的实验要求.
The genomic DNA was isolated from the leaf of Pteroceltis tatarinowii Maxim.by using the improved high salt and low pH method,improved SDS method,improved CTAB method and genomic DNA kit.The quality of the DNA was examined by agarose gel electrophoresis,spectrophotometry and PCR amplification of ISSR.The result showed that the method of improved CTAB was obviously better than the others.The DNA sample obtained by the improved CTAB method was pure and had good quality which could satisfy the need for further analysis.
出处
《安徽师范大学学报(自然科学版)》
CAS
北大核心
2011年第3期256-259,共4页
Journal of Anhui Normal University(Natural Science)
基金
国家自然科学基金(30840020
30970292)
关键词
青檀
基因组DNA
DNA提取
Pteroceltis tatarinowii Maxim
genomic DNA
DNA extraction