对不同温度酶解液制备酪蛋白磷酸肽的探讨

Discussion of Preparation of Casein Phosphopetides of Enzyme Solution at Various Temperatures

采用胰蛋白酶水解酪蛋白,可释放出促进钙吸收的酪蛋白磷酸肽。主要研究在40、50、60℃3个不同温度下,用胰蛋白酶水解酪蛋白,沉淀经干燥后,用Sephadex G-25柱层析进行分离纯化的效果。从洗脱图谱可以看出,3个温度下的CPPs洗脱体积与标准品相比基本一致,都在8 mL～40 mL处出峰,产品主要集中在峰1,说明蛋白质的肽键已基本被打断,得到了所需要的短肽。而60℃下所得CPPs产品的N/P(摩尔比)最小,纯化效果较好,是较理想的制备条件。

The casein was hydrolyzed by trypsin at three different temperatures of 40,50,60 ℃,the precipitation was separated by using Sephadex G-25.In comparison with standard,the elution volume was 8 mL-40 mL,roughly the same,which showed that peptide bonds were broken basically.The N/P（molar ratio） of CPPs at 60 ℃ was lower than other two temperatures and was an ideal preparation condition.