心脉隆对窒息新生鼠心肌NF-κB和TNF-α的影响

Effects of Xinmailong on Expression of NF-κB and TNF-α in Myocardium of Asphyxiated Neonatal Rats

目的观察窒息新生鼠应用心脉隆干预后心肌缺氧缺血损伤NF-κB和TNF-α的变化,探讨心脉隆抗未成熟心肌缺氧缺血损伤的作用及其机制。方法将96只新生SD大鼠按随机数字表法分为3组:正常对照组(n=32)、窒息组(n=32)、心脉隆干预组(n=32),各组再分3、6、12、24 h 4个时间点,每个时间点各8只;建立新生鼠窒息模型,测定新生鼠血清心肌酶(CK-MB)水平并观察心肌组织病理形态学变化,应用免疫组织化学法检测心肌NF-κB的表达、ELISE法检测心肌TNF-α的表达。结果窒息组血清CK-MB水平、心肌NF-κB和TNF-α的表达较正常对照组显著增强,以6 h达到高峰(均P<0.01),且心肌NF-κB和TNF-α表达具有正相关性(r=0.979,P<0.01);心脉隆干预组与窒息组相比,血清CK-MB水平、心肌NF-κB和TNF-α的表达均显著减弱(均P<0.01)。结论窒息新生鼠心肌组织NF-κB、TNF-α的表达增加,窒息6 h后NF-κB、TNF-α的表达达高峰;心脉隆可降低心肌NF-κB、TNF-α的表达,减轻未成熟心肌损伤。

Objective To investigate the effects of Xinmailong on myocardial injury in asphyxiated neonatal rats after hypoxia-ischemia and its mechanism through observing the influence of Xinmailong on the expression of NF-κB and TNF-α in myocardium.Methods A total of 96 neonatal Sprague-Dawley rats were randomly divided into three groups： control group（n=32）,asphyxia group（n=32） and Xinmailong group（n=32）.Rats were tested at various time points（3,6,12 and 24 hours;8 rats per time point per group）.The newborn rat model of asphyxia was induced in asphyxia group and Xinmailong group.The levels of CK-MB and the pathological changes in myocardial tissue were observed.The expression of NF-κB in the myocardium was detected by immunohistochemistry and the levels of TNF-α were determined by ELISA.Results The levels of CK-MB and the expression of NF-κB and TNF-α in asphyxia group was higher than that in control group（P0.01）.The highest levels were observed at 6 hours,and there was a positive correlation between NF-κB and TNF-α（r=0.979,P0.01）.Compared with asphyxia group,Xinmailong significantly decreased the levels of CK-MB and the expression of NF-κB and TNF-α（P0.01）.Conclusion The expression of NF-κB and TNF-α increases in myocardium of asphyxiated neonatal rats and reaches the highest levels at 6 hours.Xinmailong can protect the immature myocardium by reducing the expression of NF-κB and TNF-α in newborn rats with myocardial injury.